Respiratory Virus Pneumonia after Hematopoietic Cell Transplantation (HCT): Associations between Viral Load in Bronchoalveolar Lavage Samples, Viral RNA Detection in Serum Samples, and Clinical Outcomes of HCT
Identifieur interne : 002627 ( Main/Exploration ); précédent : 002626; suivant : 002628Respiratory Virus Pneumonia after Hematopoietic Cell Transplantation (HCT): Associations between Viral Load in Bronchoalveolar Lavage Samples, Viral RNA Detection in Serum Samples, and Clinical Outcomes of HCT
Auteurs : Angela P. Campbell [États-Unis] ; Jason W. Chien [États-Unis] ; Jane Kuypers [États-Unis] ; Janet A. Englund [États-Unis] ; Anna Wald [États-Unis] ; Katherine A. Guthrie [États-Unis] ; Lawrence Corey [États-Unis] ; Michael Boeckh [États-Unis]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2010.
Abstract
Background. Few data exist on respiratory virus quantitation in lower respiratory samples and detection in serum from hematopoietic cell transplant (HCT) recipients with respiratory virus-associated pneumonia. Methods. We retrospectively identified HCT recipients with respiratory syncytial virus (RSV), parainfluenza virus, influenza virus, metapneumovirus (MPV), and coronavirus (CoV) detected in bronchoalveolar lavage (BAL) samples, and we tested stored BAL and/or serum samples by quantitative polymerase chain reaction. Results. In 85 BAL samples from 82 patients, median viral loads were as follows: for RSV (n=35), 2.6×106 copies/mL; for parainfluenza virus (n=35), 4.9×107 copies/mL; for influenza virus (n=9), 6.8×105 copies/mL; for MPV (n=7), 3.9×107 copies/mL; and for CoV (n=4), 1.8×105 copies/mL. Quantitative viral load was not associated with mechanical ventilation or death. Viral RNA was detected in serum samples from 6 of 66 patients: 4 of 41 with RSV pneumonia, 1 with influenza B, and 1 with MPV/influenza A virus/CoV coinfection (influenza A virus and MPV RNA detected). RSV detection in serum was associated with high viral load in BAL samples (P=.05), and viral RNA detection in serum was significantly associated with death (adjusted rate ratio, 1.8; P=.02). Conclusion. Quantitative polymerase chain reaction detects high viral loads in BAL samples from HCT recipients with respiratory virus pneumonia. Viral RNA is also detectable in the serum of patients with RSV, influenza, and MPV pneumonia and may correlate with the severity of disease.
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DOI: 10.1086/651662
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Chien</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Clinical Research Division, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author><author><name sortKey="Kuypers, Jane" sort="Kuypers, Jane" uniqKey="Kuypers J" first="Jane" last="Kuypers">Jane Kuypers</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Laboratory Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Clinical Research Division, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author><author><name sortKey="Englund, Janet A" sort="Englund, Janet A" uniqKey="Englund J" first="Janet A." last="Englund">Janet A. Englund</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Pediatrics, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Clinical Research Division, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author><author><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Laboratory Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Epidemiology, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author><author><name sortKey="Guthrie, Katherine A" sort="Guthrie, Katherine A" uniqKey="Guthrie K" first="Katherine A." last="Guthrie">Katherine A. Guthrie</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Clinical Research Division, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author><author><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Laboratory Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Clinical Research Division, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author><author><name sortKey="Boeckh, Michael" sort="Boeckh, Michael" uniqKey="Boeckh M" first="Michael" last="Boeckh">Michael Boeckh</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Medicine, University of WashingtonSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Clinical Research Division, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research CenterSeattle</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date type="published">2010</date><biblScope unit="vol">201</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="1404">1404</biblScope><biblScope unit="page" to="1413">1413</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Background. Few data exist on respiratory virus quantitation in lower respiratory samples and detection in serum from hematopoietic cell transplant (HCT) recipients with respiratory virus-associated pneumonia. Methods. We retrospectively identified HCT recipients with respiratory syncytial virus (RSV), parainfluenza virus, influenza virus, metapneumovirus (MPV), and coronavirus (CoV) detected in bronchoalveolar lavage (BAL) samples, and we tested stored BAL and/or serum samples by quantitative polymerase chain reaction. Results. In 85 BAL samples from 82 patients, median viral loads were as follows: for RSV (n=35), 2.6×106 copies/mL; for parainfluenza virus (n=35), 4.9×107 copies/mL; for influenza virus (n=9), 6.8×105 copies/mL; for MPV (n=7), 3.9×107 copies/mL; and for CoV (n=4), 1.8×105 copies/mL. Quantitative viral load was not associated with mechanical ventilation or death. Viral RNA was detected in serum samples from 6 of 66 patients: 4 of 41 with RSV pneumonia, 1 with influenza B, and 1 with MPV/influenza A virus/CoV coinfection (influenza A virus and MPV RNA detected). RSV detection in serum was associated with high viral load in BAL samples (P=.05), and viral RNA detection in serum was significantly associated with death (adjusted rate ratio, 1.8; P=.02). Conclusion. Quantitative polymerase chain reaction detects high viral loads in BAL samples from HCT recipients with respiratory virus pneumonia. Viral RNA is also detectable in the serum of patients with RSV, influenza, and MPV pneumonia and may correlate with the severity of disease.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Campbell, Angela P" sort="Campbell, Angela P" uniqKey="Campbell A" first="Angela P." last="Campbell">Angela P. Campbell</name></region><name sortKey="Boeckh, Michael" sort="Boeckh, Michael" uniqKey="Boeckh M" first="Michael" last="Boeckh">Michael Boeckh</name><name sortKey="Boeckh, Michael" sort="Boeckh, Michael" uniqKey="Boeckh M" first="Michael" last="Boeckh">Michael Boeckh</name><name sortKey="Boeckh, Michael" sort="Boeckh, Michael" uniqKey="Boeckh M" first="Michael" last="Boeckh">Michael Boeckh</name><name sortKey="Campbell, Angela P" sort="Campbell, Angela P" uniqKey="Campbell A" first="Angela P." last="Campbell">Angela P. Campbell</name><name sortKey="Campbell, Angela P" sort="Campbell, Angela P" uniqKey="Campbell A" first="Angela P." last="Campbell">Angela P. Campbell</name><name sortKey="Campbell, Angela P" sort="Campbell, Angela P" uniqKey="Campbell A" first="Angela P." last="Campbell">Angela P. Campbell</name><name sortKey="Chien, Jason W" sort="Chien, Jason W" uniqKey="Chien J" first="Jason W." last="Chien">Jason W. Chien</name><name sortKey="Chien, Jason W" sort="Chien, Jason W" uniqKey="Chien J" first="Jason W." last="Chien">Jason W. Chien</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Corey, Lawrence" sort="Corey, Lawrence" uniqKey="Corey L" first="Lawrence" last="Corey">Lawrence Corey</name><name sortKey="Englund, Janet A" sort="Englund, Janet A" uniqKey="Englund J" first="Janet A." last="Englund">Janet A. Englund</name><name sortKey="Englund, Janet A" sort="Englund, Janet A" uniqKey="Englund J" first="Janet A." last="Englund">Janet A. Englund</name><name sortKey="Englund, Janet A" sort="Englund, Janet A" uniqKey="Englund J" first="Janet A." last="Englund">Janet A. Englund</name><name sortKey="Guthrie, Katherine A" sort="Guthrie, Katherine A" uniqKey="Guthrie K" first="Katherine A." last="Guthrie">Katherine A. Guthrie</name><name sortKey="Kuypers, Jane" sort="Kuypers, Jane" uniqKey="Kuypers J" first="Jane" last="Kuypers">Jane Kuypers</name><name sortKey="Kuypers, Jane" sort="Kuypers, Jane" uniqKey="Kuypers J" first="Jane" last="Kuypers">Jane Kuypers</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name><name sortKey="Wald, Anna" sort="Wald, Anna" uniqKey="Wald A" first="Anna" last="Wald">Anna Wald</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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